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rhtim 3 fc  (R&D Systems)


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    R&D Systems rhtim 3 fc
    Rhtim 3 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhtim+3+fc/Recombinant+Human+TIM-3+Fc+Chimera+Protein%2C+CF/pmc10693867__jitc___2023___007631supp001-0-147-149
    Average 92 stars, based on 13 article reviews
    rhtim 3 fc - by Bioz Stars, 2026-08
    92/100 stars

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    Figure 1. M6903 inhibited TIM-3 binding to Gal-9, PtdSer, and CEACAM1. a) Binding of huTIM-3-Fc biotin (0.5 μg/mL) to plate-bound Gal-9 (2 μg/mL) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (nM). b) Staurosporine (2 μg/mL, 18 hrs) induced apoptosis in Jurkat cells, leading to exposure of surface PtdSer. Apoptotic Jurkat cells were then incubated with rhTIM-3-Fc <t>AF647</t> pre-incubated with various concentrations of M6903 or isotype control (μg/mL), and the binding of rhTIM-3-Fc AF647 on the Jurkat cells was measured by flow cytometry (MFI). c) Binding of recombinant His-tagged CEACAM1 to plate- bound huTIM-3-Fc (0.5 μg/well) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (μg/mL). a-c) Non-linear best fit lines were generated for all plots using a Sigmoid dose-response equation and mean and standard deviation (SD) are presented.
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    Figure 1. M6903 inhibited TIM-3 binding to Gal-9, PtdSer, and CEACAM1. a) Binding of huTIM-3-Fc biotin (0.5 μg/mL) to plate-bound Gal-9 (2 μg/mL) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (nM). b) Staurosporine (2 μg/mL, 18 hrs) induced apoptosis in Jurkat cells, leading to exposure of surface PtdSer. Apoptotic Jurkat cells were then incubated with rhTIM-3-Fc <t>AF647</t> pre-incubated with various concentrations of M6903 or isotype control (μg/mL), and the binding of rhTIM-3-Fc AF647 on the Jurkat cells was measured by flow cytometry (MFI). c) Binding of recombinant His-tagged CEACAM1 to plate- bound huTIM-3-Fc (0.5 μg/well) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (μg/mL). a-c) Non-linear best fit lines were generated for all plots using a Sigmoid dose-response equation and mean and standard deviation (SD) are presented.
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    Figure 1. M6903 inhibited TIM-3 binding to Gal-9, PtdSer, and CEACAM1. a) Binding of huTIM-3-Fc biotin (0.5 μg/mL) to plate-bound Gal-9 (2 μg/mL) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (nM). b) Staurosporine (2 μg/mL, 18 hrs) induced apoptosis in Jurkat cells, leading to exposure of surface PtdSer. Apoptotic Jurkat cells were then incubated with rhTIM-3-Fc AF647 pre-incubated with various concentrations of M6903 or isotype control (μg/mL), and the binding of rhTIM-3-Fc AF647 on the Jurkat cells was measured by flow cytometry (MFI). c) Binding of recombinant His-tagged CEACAM1 to plate- bound huTIM-3-Fc (0.5 μg/well) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (μg/mL). a-c) Non-linear best fit lines were generated for all plots using a Sigmoid dose-response equation and mean and standard deviation (SD) are presented.

    Journal: OncoImmunology

    Article Title: Identification and characterization of M6903, an antagonistic anti–TIM-3 monoclonal antibody

    doi: 10.1080/2162402x.2020.1744921

    Figure Lengend Snippet: Figure 1. M6903 inhibited TIM-3 binding to Gal-9, PtdSer, and CEACAM1. a) Binding of huTIM-3-Fc biotin (0.5 μg/mL) to plate-bound Gal-9 (2 μg/mL) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (nM). b) Staurosporine (2 μg/mL, 18 hrs) induced apoptosis in Jurkat cells, leading to exposure of surface PtdSer. Apoptotic Jurkat cells were then incubated with rhTIM-3-Fc AF647 pre-incubated with various concentrations of M6903 or isotype control (μg/mL), and the binding of rhTIM-3-Fc AF647 on the Jurkat cells was measured by flow cytometry (MFI). c) Binding of recombinant His-tagged CEACAM1 to plate- bound huTIM-3-Fc (0.5 μg/well) was evaluated via ELISA after pre-incubation with serial dilutions of M6903 or isotype control (μg/mL). a-c) Non-linear best fit lines were generated for all plots using a Sigmoid dose-response equation and mean and standard deviation (SD) are presented.

    Article Snippet: Apoptotic cells from each well were treated with 5% human AB serum (Valley Biomedical, HP1022) at room temperature for 1 hour. rhTIM-3-Fc AF647 (R&D Systems, 2365-TM; final concentration 2 μg/ mL) was pre-incubated with 1:3 serial dilutions of either M6903 or an anti-HEL IgG2 isotype control (EMD Serono, A15-125-1, starting at 60 μg/mL and diluting to 0.027 μg/mL final concentration) on ice for 30 minutes.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Control, Flow Cytometry, Recombinant, Generated, Standard Deviation